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Topics - alysiamandato

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Hello all, and thank you for this easy to use software!

I have extended my DNA duplex using the instructions in the forum post This was very helpful. I can provide my code if necessary.

I am having an issue when I visualize, though. I followed the steps in the post and repeated the steps to ensure I did them correctly, but it seems that there are some breaks in the backbone structure. I am wondering if there is anyway to fix this using 3DNA or if anyone has ran into this problem before. I've attached an image of what I mean.

Please let me know if there is anything I can provide.

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Created and maintained by Dr. Xiang-Jun Lu [律祥俊] (
The Bussemaker Laboratory at the Department of Biological Sciences, Columbia University.