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Author Topic: Supplementary Figure 1 -- four base triplets in yeast phenylalanine tRNA (1ehz)  (Read 1704 times)

Offline xiangjun

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"four base triplets in tRNA (1ehz)" title="four base triplets in tRNA (1ehz)"

Figure S1: The four base triplets and associated hydrogen bonds (dashed lines) detected by DSSR in yeast tRNAPhe (PDB id: 1ehz). (A) UAA (U8,A14,A21), with a reverse Hoogsteen U8–A14 pair. (B) AUA (A9,U12,A23). (C) gCG (2MG10,C25,G45). (D) CGg (C13,G22,7MG46). Here, the bases in each triplet are listed in sequential order, with the one-letter shorthand form followed by more detailed identifiers in parentheses. In (C) and (D), the lower case ‘g’ represents the shortened name for modified guanine nucleotides 2MG10 and 7MG46, respectively. Note that the triplets and hydrogen bonds match those originally reported by Quigley and Rich for yeast tRNAPhe.

Starting from "1ehz.pdb" downloaded from RCSB PDB, here is the complete script to get each of the four base-triplet images in png format.

Code: Bash
  1. x3dna-dssr -i=1ehz.pdb -o=1ehz.out --prefix=1ehz
  3. ex_str -1 1ehz-multiplets.pdb 1ehz-m1.pdb
  4. x3dna-dssr -i=1ehz-m1.pdb -o=1ehz-m1.pml --hbfile-pymol
  5. pymol -qkc 1ehz-m1.pml
  6. convert -trim +repage -border 10 -bordercolor white 1ehz-m1-pymol.png 1ehz-m1.png
  8. ex_str -2 1ehz-multiplets.pdb 1ehz-m2.pdb
  9. x3dna-dssr -i=1ehz-m2.pdb -o=1ehz-m2.pml --hbfile-pymol
  10. pymol -qkc 1ehz-m2.pml
  11. convert -trim +repage -border 10 -bordercolor white 1ehz-m2-pymol.png 1ehz-m2.png
  13. ex_str -3 1ehz-multiplets.pdb 1ehz-m3.pdb
  14. x3dna-dssr -i=1ehz-m3.pdb -o=1ehz-m3.pml --hbfile-pymol
  15. pymol -qkc 1ehz-m3.pml
  16. convert -trim +repage -border 10 -bordercolor white 1ehz-m3-pymol.png 1ehz-m3.png
  18. ex_str -4 1ehz-multiplets.pdb 1ehz-m4.pdb
  19. x3dna-dssr -i=1ehz-m4.pdb -o=1ehz-m4.pml --hbfile-pymol
  20. pymol -qkc 1ehz-m4.pml
  21. convert -trim +repage -border 10 -bordercolor white 1ehz-m4-pymol.png 1ehz-m4.png
  • The --prefix option makes the auxiliary files having a specified prefix instead of the default "dssr". For example, "dssr-multiplets.pdb" becomes "1ehz-multiplets.pdb".
  • The ex_str utility program is from the 3DNA distribution. It is used to extract a specific model from a MODEL/ENDMDL ensemble.
  • The DSSR --hbfile-pymol option is used to generate a .pml file with all required settings for rendering in PyMOL.
  • The convert  program is from ImageMagick that is used here to trim extra white boundaries.
  • The multiplet-png images (here four triplets) were combined using InkScape, and annotated, to get the final illustration.
  • For completeness, here is the tarball file containing all the data files and the script ("tasks"): supp-fig1-1ehz-multiplets.tar.gz

Here is a sample image generated with the above script:
« Last Edit: August 05, 2015, 05:48:21 pm by xiangjun »
Dr. Xiang-Jun Lu [律祥俊]


Created and maintained by Dr. Xiang-Jun Lu[律祥俊]· Supported by the NIH grant R01GM096889 · Dr. Lu is currently a member of the Bussemaker Laboratory at the Department of Biological Sciences, Columbia University. The project is in collabration with the Olson Laborarory at Rutgers where 3DNA got started.