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Author Topic: Figure 5 -- analysis of the SAM-I riboswitch (2gis)  (Read 1359 times)

Offline xiangjun

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Figure 5 -- analysis of the SAM-I riboswitch (2gis)
« on: July 08, 2015, 08:43:50 pm »
"DSSR analysis of the SAM-I riboswitch (2gis)" title="DSSR analysis of the SAM-I riboswitch (2gis)"

Figure 5: DSSR pinpoints a linchpin-like U64–A85 pair that is shared by a four-way and a five-way junction loop in the S-adenosyl methionine I riboswitch (PDB id: 2gis (45)). (A) DSSR identifies two junction loops (right): a [4,0,3,0] four-way junction loop (red) and a [1,0,2,0,0] five-way junction loop (blue), which share a common side, i.e., the isolated U64–A85 pair (left). (B) The linear secondary structure diagram, annotated with DSSR-derived dot-bracket notation, depicts the pathways of the two junction loops. The four-way loop runs from C8 (*), follows the red arrows to the right, and returns along the outer G86→C8 arc. The five-way loop starts at G23 (*), moves to the right following the blue arrows along two arcs (C25→G68 and C69→G82), and returns to the start along three arcs (A85→U64, C65→G28, C29→G23). Note that the shared U64–A85 arc is traversed twice, from left to right along the four-way junction loop, and right to left along the five-way junction loop. (C) The U64–A85 pair is stabilized by base-stacking interactions in a way strikingly similar to the G2–C74 linchpin pair in the viral tRNA mimic (see Figure 3), and may also be regarded as a ‘linchpin’. These two images take advantage of unique visualization features within 3DNA/DSSR, including the capability to orient different molecules in a common frame (here, the frames of the linchpin pairs with the minor-groove edges facing the viewer) and to represent bases as color-coded rectangular blocks.

Here is the tarball (fig5-SAM-I-2gis.tar.gz) with the script and all related data files.

The content of the full script (named tasks) is shown below. Please see also notes for "Figure 2 -- analysis of the yeast phenylalanine tRNA (1ehz)".

Code: Bash
  1. # Step #1 -- reorient SAM-I riboswitch into the most extended view
  2. pdb_frag A 1:94 A 301 2gis.pdb 2gis-nts.pdb
  3. rotate_mol 2gis-nts.pdb temp
  4. rotate_mol -r=2gis.rot temp 2gis-ok.pdb
  5. x3dna-dssr -i=2gis-ok.pdb --prefix=2gis-ok -o=2gis-ok.out
  7. # To get the result illustrated in panel B, load '2gis-ok-2ndstrs.ct'
  8. # or '2gis-ok-2ndstrs.dbn' into VARNA to draw the linear secondary
  9. # structure diagram, exported as .svg for annotation in Inkscape.
  11. # Step #2 -- get the cartoon-block representation, with fitted helices
  12. x3dna-dssr -i=2gis-ok.pdb --helical-axis -o=temp
  13. \mv dssr-helicalAxes.pdb 2gis-ok-helices.pdb
  14. x3dna-dssr -i=2gis-ok.pdb --block-file -o=2gis-ok-blocks.r3d
  16. # Step #3 -- simplified representation of the [4,0,3,0] 4-way
  17. #            and [1,0,2,0,0] 5-way junctions in 3D
  18. #   note the '--raw-xyz' option: it keeps the original coordinates
  19. x3dna-dssr -i=2gis-ok.pdb --raw-xyz --simple-junction -o=temp
  20. \mv dssr-simplifiedJcts.pdb 2gis-ok-jctx.pdb
  21. ex_str -1 2gis-ok-jctx.pdb 2gis-ok-jct.pdb  # 4-way junction
  22. ex_str -2 2gis-ok-jctx.pdb 2gis-ok-jct2.pdb # 5-way junction
  24. # see file: 2gis-ok-jct.pml
  25. pymol -qkc 2gis-ok-jct.pml
  26. convert -trim +repage -border 10 -bordercolor white 2gis-ok-jct-pymol.png 2gis-ok-jct.png
  28. # see file: 2gis-ok-full.pml (cartoon-block with the schematic
  29. #                            junctions overlaid) -- panel A
  30. pymol -qkc 2gis-ok-full.pml
  31. convert -trim +repage -border 10 -bordercolor white 2gis-ok-full-pymol.png 2gis-ok-full.png
  33. # Step #4 -- pair U64-A85 stablized by base-stacking interactions
  34. pdb_frag A 63:65 A 82:86 2gis-ok.pdb 2gis-ok-UA.pdb
  35. x3dna-dssr -i=2gis-ok-UA.pdb --block-file -o=2gis-ok-UA-blocks.r3d
  36. # see file: 2gis-ok-UA.pml
  37. pymol -qkc 2gis-ok-UA.pml
  38. convert -trim +repage -border 10 -bordercolor white 2gis-ok-UA-pymol.png 2gis-ok-UA.png
  40. # Step #5 -- the U64-A85 isolated pair is linchpin-like (panel C)
  41. x3dna-dssr -i=2gis-ok-UA.pdb --frame=A.64:wc+edge -o=2gis-stacks.pdb
  42. x3dna-dssr -i=2gis-stacks.pdb --block-file -o=2gis-stacks-blocks.r3d
  43. # see file: 2gis-stacks.pml
  44. pymol -qkc 2gis-stacks.pml
  45. convert -trim +repage -border 10 -bordercolor white 2gis-stacks-pymol.png 2gis-stacks.png
  47. x3dna-dssr -i=4p5j-ok-linchpin.pdb --frame=A.74:wc+edge -o=4p5j-stacks.pdb
  48. x3dna-dssr -i=4p5j-stacks.pdb --block-file -o=4p5j-stacks-blocks.r3d
  49. # see file: 4p5j-stacks.pml
  50. pymol -qkc 4p5j-stacks.pml
  51. convert -trim +repage -border 10 -bordercolor white 4p5j-stacks-pymol.png 4p5j-stacks.png

Here are the images generated from the above script:

« Last Edit: August 05, 2015, 05:39:06 pm by xiangjun »
Dr. Xiang-Jun Lu [律祥俊]


Created and maintained by Dr. Xiang-Jun Lu[律祥俊]· Supported by the NIH grant R01GM096889 · Dr. Lu is currently a member of the Bussemaker Laboratory at the Department of Biological Sciences, Columbia University. The project is in collabration with the Olson Laborarory at Rutgers where 3DNA got started.