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Messages - tpc2005

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1
MD simulations / Re: How to fix the phosphate on the DNA backbone
« on: November 20, 2016, 03:31:26 am »
Thanks so much for the quick reply! I will try PHENIX!

2
MD simulations / Re: How to fix the phosphate on the DNA backbone
« on: November 19, 2016, 03:08:07 pm »
Please see the attachment of pdb file.
The two breaks happen on (chain D:position 6, chain D:position 7) and (chain C:position 20, chain M:position 7).

Appreciate!

3
MD simulations / How to fix the phosphate on the DNA backbone
« on: November 19, 2016, 02:33:19 pm »
Hi,

I have two DNA structures from PDB. I designed a five-base-pair linker to connect these two DNA structures by using BIOVIA Discovery Studio. I've almost done it but found there are two breaking points on the phosphate backbone. Does anyone know how to fix it? Thanks so much!

Best,
Lester

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Created and maintained by Dr. Xiang-Jun Lu [律祥俊] (xiangjun@x3dna.org)
The Bussemaker Laboratory at the Department of Biological Sciences, Columbia University.