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General discussions (Q&As) / bending angle of DNA
« on: August 04, 2010, 10:21:20 pm »
Dear all,
We are working on Sox proteins that bind the minor groove of DNA and induce a rather drastic bend. 3DNA is of fantastic help to analyze base-pair and base step parameters. However, it's common standard to report an actual overall bending angle. It's surely possible to estimate such a bending angle from structural models and report a rough value - we have done so in the past. But I am wondering whether there is a more accurate way of defining this angle (i.e. by defining a kink point and helical axis extending from there).
Regards,
Ralf
We are working on Sox proteins that bind the minor groove of DNA and induce a rather drastic bend. 3DNA is of fantastic help to analyze base-pair and base step parameters. However, it's common standard to report an actual overall bending angle. It's surely possible to estimate such a bending angle from structural models and report a rough value - we have done so in the past. But I am wondering whether there is a more accurate way of defining this angle (i.e. by defining a kink point and helical axis extending from there).
Regards,
Ralf