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1

General discussions (Q&As) / Re: Noobie question on building triplex DNA

« on: May 01, 2009, 10:43:49 am »

Thank you for the advice!

I have been looking for some open-source software that will allow me to build a 3D DNA model and save the coordinates in pdb format. I have yet to find software that will meet these needs. With the standard crystallographic software you can make mutations easily, however adding in new residues gets tricky.

Thanks again for your assistance,

Jason

2

General discussions (Q&As) / Noobie question on building triplex DNA

« on: April 30, 2009, 12:37:51 pm »

Hi everbody!

Sorry for the noobie question, but is it possible to build a triplex DNA from scratch? If not, is it possible to take a .pdb file of a triplex DNA and mutate it to my own sequence? This would involve insertion of an extra residue in the loop region. I would later like to save the new coordinates in .pdb format for molecular dynamics studies.

Thanks in advance for any guidance!

Jason