Messages

1

General discussions (Q&As) / Re: Problems with mutations on DNA

« on: February 14, 2011, 04:54:28 am »

Thank you xiangjun for your very exhaustive explanation..
I need to perform a lot of mutation so perhaps I need to make a script to make this process automatic..
Thank you again for all the suggestions and I promise you to update about my progresses..

2

General discussions (Q&As) / Problems with mutations on DNA

« on: February 11, 2011, 10:09:40 am »

Hi 3DNA users and xiangjun in particular for your effort to make possible to use 3DNA and to discuss about possible problems and solutions.
I'm trying to use 3DNA to mutate one base pair of a protein-DNA complex, but I don't understand how to make this operation, if possible.
I have tried the examples and the tutorial, but there I haven't found any similar example.
I have just the template structure with the double strand sequence GCGT and I want, for example, to mutate the base pair CG with TA but I haven't found a similar example in the tutorial or in the examples.                                                                                   CGCA
Anyone uses/used 3DNA for this particular operation? COuld you please give me an advice about this work?
Thank you!
Rubben