Recent Posts

1

RNA structures (DSSR) / Re: overlapping area calculation - stacking interactions

« Last post by Agnieszka on Yesterday at 12:42:34 pm »

Yes, please. That would be convenient to have it as downloadable wDSSR file.
Thank you,
Agnieszka

2

RNA structures (DSSR) / Re: overlapping area calculation - stacking interactions

« Last post by xiangjun on Yesterday at 11:37:00 am »

Hi Agnieszka,

Thank you for using 3DNA/DSSR and for sharing your feedback.

The new wDSSR website introduces several modernized features, though a few specialized analyses from the legacy 3DNA 2.0 web server were initially omitted to keep the interface streamlined. Based on recent user requests, we have already restored backbone torsion angles to the wDSSR platform.

Regarding the base-pair overlapping area: this metric is calculated by the underlying DSSR engine but is not yet rendered on the wDSSR web server. In the meantime, you can obtain these overlapping areas directly by running the DSSR command-line program locally.

We are happy to integrate this feature into the web platform. Which format would best fit your workflow? For instance, would adding a dedicated section to the downloadable wDSSR output file be sufficient?

Code: [Select]

Overlap area in Angstrom^2 between polygons defined by atoms on successive
bases. Polygons projected in the mean plane of the designed base-pair step.

Values in parentheses measure the overlap of base ring atoms only. Those
outside parentheses include exocyclic atoms on the ring. Intra- and
inter-strand overlap is designated according to the following diagram:

                    i2  3'      5' j2
                       /|\      |
                        |       |
               Strand I |       | II
                        |       |
                        |       |
                        |      \|/
                    i1  5'      3' j1

     step      i1-i2        i1-j2        j1-i2        j1-j2        sum
   1 CG/CG  1.48( 0.00)  0.00( 0.00)  0.97( 0.00)  0.94( 0.00)  3.39( 0.00)
   2 GC/GC  2.95( 0.63)  0.00( 0.00)  0.00( 0.00)  0.96( 0.04)  3.91( 0.68)
   3 CG/CG  2.66( 0.00)  0.00( 0.00)  0.15( 0.00)  3.44( 0.21)  6.25( 0.21)
   4 GA/TC  3.94( 1.11)  0.00( 0.00)  0.00( 0.00)  4.17( 0.78)  8.11( 1.88)
   5 AA/TT  3.51( 2.16)  0.00( 0.00)  0.00( 0.00)  5.09( 0.08)  8.60( 2.24)
   6 AT/AT  5.91( 2.14)  0.00( 0.00)  0.00( 0.00)  5.65( 1.89) 11.56( 4.03)
   7 TT/AA  5.08( 0.11)  0.00( 0.00)  0.00( 0.00)  3.68( 2.39)  8.76( 2.51)
   8 TC/GA  2.20( 0.00)  0.00( 0.00)  0.00( 0.00)  4.98( 2.19)  7.18( 2.19)
   9 CG/CG  5.50( 1.17)  0.00( 0.00)  0.01( 0.00)  1.56( 0.00)  7.06( 1.17)
  10 GC/GC  0.57( 0.00)  0.00( 0.00)  0.00( 0.00)  5.32( 2.35)  5.89( 2.35)
  11 CG/CG  0.73( 0.00)  0.00( 0.00)  0.54( 0.00)  1.98( 0.03)  3.25( 0.03)
Best regards,

Xiang-Jun

3

RNA structures (DSSR) / overlapping area calculation - stacking interactions

« Last post by Agnieszka on Yesterday at 08:29:21 am »

Hi,

I used new online xdna-dssr server and got calculated parmeters. However I can't see anymore the calculation of overlapping area as used to be in older version which I liked it very much. Is this option still available?

Agnieszka

4

Feature requests / Re: where are the torsion angles?

« Last post by xiangjun on June 01, 2026, 10:54:40 pm »

As a follow up, the wDSSR web server has been updated to include a link to torsion angles in the "Analyze" module. See an example output for PDB entry 1msy.

5

Feature requests / Re: where are the torsion angles?

« Last post by xiangjun on May 28, 2026, 10:25:56 pm »

Hi,

Thank you for your question. DSSR calculates a wide range of structural features, including backbone torsion angles. To keep the initial release of the wDSSR web server streamlined and accessible, some of these detailed parameters were intentionally omitted from the default display. In the meantime, you can obtain complete torsion angle profiles using the DSSR command-line tool, as detailed in the DSSR User Manual.

We can update wDSSR to make this data available on the web interface. Would you prefer to have the torsion angles included in a downloadable JSON output file, or displayed explicitly in a new section on the results page?

Best regards,

Xiang-Jun

6

Feature requests / where are the torsion angles?

« Last post by annawacker on May 28, 2026, 10:10:02 am »

I am missing the torsion angle analysis on the new web3DNA server. why is it gone?

7

FAQs / Re: Where to download x3DNA

« Last post by xiangjun on May 11, 2026, 05:09:54 pm »

Hi,

Thank you for joining the 3DNA/DSSR community. For security, initial posts from non-EDU addresses require a one-time approval. This has been completed, and your download link is now active.

Best regards,

Xiang-Jun

8

FAQs / Re: Where to download x3DNA

« Last post by layne on May 11, 2026, 04:51:28 pm »

Hi,

I also got the confirmation email and successfully logged in this forum, but I cannt find the download page. Could you help me check it?

Thank you!

9

FAQs / Re: Where to download x3DNA

« Last post by amtblcd on May 05, 2026, 02:42:48 am »

Hi Xiang-Jun,

 Got it, thanks.

Best regards,
Changdong

10

FAQs / Re: Where to download x3DNA

« Last post by xiangjun on April 27, 2026, 10:24:39 am »

Hi Changdong,

Thank you for the follow-up.

I would like to clarify that the licensing for DSSR is managed by Columbia Technology Ventures (CTVs). While DSSR has a large global community with hundreds of academic users, licenses are generally not issued to institutions in certain countries due to institutional compliance and administrative policies. Unfortunately, this means that even if an academic request is submitted, it may be restricted based on the location of the host institution.

However, you can still access DSSR through the following options:

• DSSR v1.9.10-2020apr23: This version is available for download directly from the 3DNA Forum. It corresponds to the paper "DSSR-enabled innovative schematics of 3D nucleic acid structures with PyMOL" (2020) published in Nucleic Acids Research.
• wDSSR: You can use the recently released web interface for DSSR at https://web.x3dna-dssr.org/.
  

I appreciate your interest in the software and your understanding of these constraints.

Best regards,

Xiang-Jun