Tools for connecting two DNAs into one

[xclin]

Hi,

I am a new user to 3DNA. I have two separate DNAs and would like to connect them into one. I am wondering if 3DNA has tools for this purpose?

Thanks,
Xingcheng

[xiangjun]

Hi Xingcheng,

Could you please provide a concrete example to illustrate what you mean by connecting two DNA molecules into one?

Thanks,

Xiang-Jun

[xclin]

Hi,

Thanks for your kind response.

What I was trying to do is to connect two separate DNAs, such as two polyA DNAs into one longer DNA. I just realized that the instruction on extending the length of one DNA as illustrated here
http://forum.x3dna.org/users-contributions/build-dna-bulges-and-extend-dna-duplex-at-both-terminals-via-3dna/
can be useful for my purpose.

Best,
Xingcheng

[xiangjun]

Have a try, and report back any issues you experience. As a general rule, please remember to be specific with your questions.

Best regards,

Xiang-Jun