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General discussions (Q&As) / Tolerance of mutate_bases
« on: November 05, 2016, 12:53:08 pm »
Dear mr. Xiang-Jun,
I am a Bioinformatics graduate student and I am trying to construct an RNA structure because there
is not an available experimentally derived one. I have found a similar structure and I want to use mutate_bases
to change some of the bases. Also, I intend to perform energy minimization to the resulting structure after the changes.
I would like to know about the limits of this strategy. I wondered if there are areas in the structure
that are "more sensitive" to such changes. Can I make consecutive mutations? Can I change a big area?
Thank you in advance.
Regards,
Panos
I am a Bioinformatics graduate student and I am trying to construct an RNA structure because there
is not an available experimentally derived one. I have found a similar structure and I want to use mutate_bases
to change some of the bases. Also, I intend to perform energy minimization to the resulting structure after the changes.
I would like to know about the limits of this strategy. I wondered if there are areas in the structure
that are "more sensitive" to such changes. Can I make consecutive mutations? Can I change a big area?
Thank you in advance.
Regards,
Panos