Messages

1

General discussions (Q&As) / Re: RNA overwinding

« on: June 12, 2014, 11:10:00 am »

Thanks for your insights Xiang-Jun,

however, I did look at the twist values of the 2 helices in question and there do not seem to be gross differences, or are differences of about 1deg already significant?
I noticed that the RNA helix gets wider upon the screw-like motion that I observe in my structures. This then leads to RNA compression by a one base pair step.
Isnt it possible that the twist angles stay the same when the helix gets wider during screw-like compression??

Thanks for your help.

Claus

2

General discussions (Q&As) / RNA overwinding

« on: June 11, 2014, 04:42:38 pm »

Hi,
I would like to quantify overwinding for an RNA double-helical structure in pdb-format.
How can I achieve that with the 3DNA output?

Thanks for your help,


Claus D. Kuhn
Cold Spring Harbor Laboratory