3DNA Forum

Questions and answers => General discussions (Q&As) => Topic started by: xzhang1999 on May 05, 2014, 02:27:56 pm

Notice: The 3DNA Forum is now a read-only archive. See the announcement.
All active support and discussions have moved to GitHub Discussions!

Title: how to mutate multiple nucleotides with mutate_bases?
Post by: xzhang1999 on May 05, 2014, 02:27:56 pm

Hi,

How can I use mutate_bases to build a homology model using a solved rRNA structure? For example, I want to mutate nucleotides 1-10 in the solved structure with a new sequence "AUAUAUAUAU"?

Thanks much for advice.

Xing

Title: Re: how to mutate multiple nucleotides with mutate_bases?
Post by: xiangjun on May 05, 2014, 03:12:25 pm

Hi Xiang,

Does not the post on mutate_bases (http://forum.x3dna.org/programs/mutate_bases/) help?

Note that you can use the "-e" option to get a list of all nucleotides in your structure, and then modify the output file to suit your needs.

If you still have problems, please be concrete with a specific example.

HTH,

Xiang-Jun

This forum is archived. Please post new questions on GitHub Discussions!