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Questions and answers => General discussions (Q&As) => Topic started by: xzhang1999 on May 05, 2014, 02:27:56 pm

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Title: how to mutate multiple nucleotides with mutate_bases?
Post by: xzhang1999 on May 05, 2014, 02:27:56 pm

Hi,

How can I use mutate_bases to build a homology model using a solved rRNA structure? For example, I want to mutate nucleotides 1-10 in the solved structure with a new sequence "AUAUAUAUAU"?

Thanks much for advice.

Xing

Title: Re: how to mutate multiple nucleotides with mutate_bases?
Post by: xiangjun on May 05, 2014, 03:12:25 pm

Hi Xiang,

Does not the post on mutate_bases (http://forum.x3dna.org/programs/mutate_bases/) help?

Note that you can use the "-e" option to get a list of all nucleotides in your structure, and then modify the output file to suit your needs.

If you still have problems, please be concrete with a specific example.

HTH,

Xiang-Jun

Funded by the NIH R24GM153869 grant on X3DNA-DSSR, an NIGMS National Resource for Structural Bioinformatics of Nucleic Acids

Created and maintained by Dr. Xiang-Jun Lu, Department of Biological Sciences, Columbia University