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Author Topic: Tools for connecting two DNAs into one  (Read 108 times)

Offline xclin

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Tools for connecting two DNAs into one
« on: April 21, 2019, 04:11:55 pm »
Hi,

I am a new user to 3DNA. I have two separate DNAs and would like to connect them into one. I am wondering if 3DNA has tools for this purpose?

Thanks,
Xingcheng

Offline xiangjun

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Re: Tools for connecting two DNAs into one
« Reply #1 on: April 21, 2019, 05:38:37 pm »
Hi Xingcheng,

Could you please provide a concrete example to illustrate what you mean by connecting two DNA molecules into one?

Thanks,

Xiang-Jun
Dr. Xiang-Jun Lu [律祥俊]
Email: xiangjun@x3dna.org
Homepage: http://x3dna.org/
Forum: http://forum.x3dna.org/

Offline xclin

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Re: Tools for connecting two DNAs into one
« Reply #2 on: April 21, 2019, 05:57:14 pm »
Hi,

Thanks for your kind response.

What I was trying to do is to connect two separate DNAs, such as two polyA DNAs into one longer DNA. I just realized that the instruction on extending the length of one DNA as illustrated here
http://forum.x3dna.org/users-contributions/build-dna-bulges-and-extend-dna-duplex-at-both-terminals-via-3dna/
can be useful for my purpose.

Best,
Xingcheng

Offline xiangjun

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Re: Tools for connecting two DNAs into one
« Reply #3 on: April 21, 2019, 06:09:46 pm »
Have a try, and report back any issues you experience. As a general rule, please remember to be specific with your questions.

Best regards,

Xiang-Jun
Dr. Xiang-Jun Lu [律祥俊]
Email: xiangjun@x3dna.org
Homepage: http://x3dna.org/
Forum: http://forum.x3dna.org/

 

Created and maintained by Dr. Xiang-Jun Lu [律祥俊], Principal Investigator of the NIH grant R01GM096889
Dr. Lu is currently affiliated with the Bussemaker Laboratory at the Department of Biological Sciences, Columbia University.