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Author Topic: rebuilding using Modified bases  (Read 12020 times)

Offline kailsen

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rebuilding using Modified bases
« on: March 17, 2016, 01:07:12 pm »
Hi ,

I'm  using rebuild option for regenerating my sequence.
I wanted to change the nucleotide (base+sugar+backbone) coordinates used to a modified nucleotide.
So as to regenerate a new duplex. Which library file used by rebuild module?

Best,
Kailsen

Offline xiangjun

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Re: rebuilding using Modified bases
« Reply #1 on: March 17, 2016, 01:22:49 pm »
Hi Kailsen,

It is possible to generate a duplex with modified nucleotides, using a combination of 3DNA programs (including std_base, rebuild, fiber, mutate_bases etc). For example, you could run rebuild with the primary sequence, and then mutate_bases to get the desired modifications. Please provide a concrete example so we can discuss details.

Xiang-Jun 

Offline kailsen

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Re: rebuilding using Modified bases
« Reply #2 on: March 17, 2016, 01:34:52 pm »
Hi 
Thanks for the quick reply.
One way to do will be as u suggested.
But I also want to modify backbone atoms.
I got the answer from your earlier post.

http://forum.x3dna.org/faqs/how-do-i-build-nucleic-acid-structures-with-sugar-phosphate-backbone/msg784/#msg784
Thanks once again,

Best,
kailsen

 

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