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Questions and answers => General discussions (Q&As) => Topic started by: laurap on November 17, 2011, 02:43:45 am

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Title: modelling ssDNA or ssRNA
Post by: laurap on November 17, 2011, 02:43:45 am

Dear Xiang-Jun,

I would like to model an extended conformation of an ssDNA (GT(x15) sequence) and ssRNA (GU(x12) sequence). I mean, is it possible to model single strands without helical configurations (not like the half of a duplex) using 3DNA server/package?

Thanks and regards,

Laura

Title: Re: modelling ssDNA or ssRNA
Post by: xiangjun on November 17, 2011, 07:38:23 am

Hi Laura,

Thanks for your interest in 3DNA and for posting on the forum.

I understand what you mean, but I do not think 3DNA (or other tools I know of) can provide you with a straight answer. It all depends -- what specifically is your definition of "an extended conformation", i.e., "single strands without helical configurations"? If you have a starting model, mutate_bases (http://forum.x3dna.org/programs/mutate_bases/) and analyze/rebuild/frame_mol in 3DNA may help.

As always, I am willing to extend 3DNA in ways that make sense to me and are useful to the community. Currently, 3-dimensional nucleic acid structure modeling is one aspect I am interested in.

Xiang-Jun

Created and maintained by Dr. Xiang-Jun Lu [律祥俊] (xiangjun@x3dna.org)
The Bussemaker Laboratory at the Department of Biological Sciences, Columbia University.