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Miscellaneous items > FAQs

How to build a single-stranded RNA structure of arbitrary sequence?

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xiangjun:
As of v2.1, the fiber utility has several new options that make building single-stranded RNA structures of arbitrary sequence a snap:

* -s for single-strand
* -r for RNA structure
* -seq for specifying arbitrary sequence directly on the command lineFor example, to generate a single-stranded RNA structure of sequence 'AUUGGUUC', do the following:

--- Code: ---fiber -s -r -seq=AUUGGUUC ss-RNA.pdb
# the -s and -r options can be combined as -sr or -rs
fiber -sr -seq=AUUGGUUC ss-RNA.pdb
--- End code ---

Technically, the RNA model is based on A-DNA model (#1 in the list), with the O2' atom attached on the sugar, and T replaced by U. The -s option simply extracts the leading strand from the default duplex.

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Created and maintained by Dr. Xiang-Jun Lu [律祥俊] (xiangjun@x3dna.org)
The Bussemaker Laboratory at the Department of Biological Sciences, Columbia University.