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Documentation => FAQs => Topic started by: xiangjun on March 20, 2012, 02:35:25 pm

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Title: How to build a single-stranded RNA structure of arbitrary sequence?
Post by: xiangjun on March 20, 2012, 02:35:25 pm
As of v2.1, the fiber utility has several new options that make building single-stranded RNA structures of arbitrary sequence a snap:
For example, to generate a single-stranded RNA structure of sequence 'AUUGGUUC', do the following:
Code: [Select]
fiber -s -r -seq=AUUGGUUC ss-RNA.pdb
# the -s and -r options can be combined as -sr or -rs
fiber -sr -seq=AUUGGUUC ss-RNA.pdb

Technically, the RNA model is based on A-DNA model (#1 in the list), with the O2' atom attached on the sugar, and T replaced by U. The -s option simply extracts the leading strand from the default duplex.

Created and maintained by Dr. Xiang-Jun Lu [律祥俊], Principal Investigator of the NIH grant R01GM096889
Dr. Lu is currently affiliated with the Bussemaker Laboratory at the Department of Biological Sciences, Columbia University.